この場合には英語を使う方がいいかも。
Title: Unable to recognize my audio interface.
"Even though my audio interface is connected and articulates during playback, it is not recognizable by Studio One 3 Prime and the recording button is not highlighted and triggerable. Reboot and Reinstallation could not cope with this issue. Wondering what shall I do next."
I guess you are using Windows version of Studio One with an audio interface which has lack of necessary ASIO support.