Studio One is just a DAW that accepts inputs and routes outputs. To accomplish what you are trying to do, you will need to route an audio output from an interface into an input on the same interface. Some audio interfaces offer the option to loop back audio inside of the control panel for the driver, but not many. The good news is that there is a way to do it with a 3rd-party software app. One of which is called "Banana." Here is a link to Banana's web site.
If you are on a Mac, here is an article in our PreSonus Answers system:
Here is a video from a 3rd-party that explains how to route your audio with cables to record Skype calls.