-Try going into Documents>Studio One>Songs>Your song name and delete the "cache" folder. Do this with Studio One closed and then re-load the song.
-Make sure that you are running the latest build of your version of Studio One that you are working with.
-Try removing settings files from the program and re-configure.
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Go to Help>Open Settings folder.
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Copy the contents of the folder over to a folder on your desktop.
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Close S1 and delete the contents of the settings folder with the exception of the user.license file as well as the "Extensions" folder.
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Open S1 and go to Studio One>Options (Preferences on a Mac) to reconfigure your settings.
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Test to see if the problem is resolved. If it is not, please feel free to copy your settings files back over from the backup that you created in step 2.
*If you are missing presets or Sound Sets after doing this, simply double-click one of your Sound Sets under Documents>Studio One>Sound Sets.
-If the issue persists, try re-installing the driver for your audio interface. Make sure that your song is running at a sample rate that is supported by your device.