-If you are working with external files that you have dragged into the program, you will want to go into Studio One>Options>Locations>User Data and check the box to "ask to copy external files when saving." This way, your external files will be saved to Documents\Studio One\Songs\Your Song Name\media when saving the song. That is if you have not changed the User Data folder location.
-Go into Studio One>Options>Locations>User Data and make sure that you are not pointing to One Drive. If you are using One Drive on your machine, you may want to opt out of the Documents>Studio One directory. Here is an article from Microsoft that explains how to manage One Drive.
-If you are using a different Cloud Storage solution, try disabling it while you are working with audio.
-Open the Event Viewer for Windows, go into the "System" section of "Windows Logs" and make sure that you do not have any errors with Event ID number 7 in them. This would indicate bad blocks on your hard drive.
-If the problem persists, you may want to submit a support ticket by logging into your my.presonus.com account and clicking on "Support."