5/16/2020 I am using Studio One 3 Professional . This addresses one specific setting but it may help.
Start Studio One 3 (or your version)
Select Studio One, then Options, then the Audio Setup tab
The Options box in the lower left corner needs to be selected
Select the Processing tab
In the Process Precision drop down box, select Single (32 bit) – not Double (64 bit).
My system is a 64 bit system but it still doesn't work.
The sound should work.
I do not have my Presonus Audio Box USB connected.