The issue is that your Series III Console is set to Stagebox mode or Monitor Mix mode- and should be in “Standalone” mode.
Are you using a Series III Rack mixer with your Console?
If so, below are two methods of re-enabling the Main Mix layer.
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Network method:
Use this method if you have both units on a wireless router via their respective control ports
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On the console, set the StudioLive 32R to stand-alone and apply.
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Connect to the 32R using UC Surface. Click the gear in the upper right corner.
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In the Network tab, set the StudioLive 32 to stand-alone and apply.
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On the console, you should now be able to press the main mix button.
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Still on the console, set the 32R to stagebox mode.
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Set your desired channels to network.
You should now be able to select mixes and go back to select the main mix.
USB method:
This will require a laptop with Universal Control installed, a USB cable and physical access to the 32R.
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On the console, set the StudioLive 32R to stand-alone and apply.
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Connect a laptop to the 32R via USB and open Universal Control. Click the gear in the upper right corner.
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In the Network tab, set the StudioLive 32 to stand-alone and apply.
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On the console, you should now be able to press the main mix button.
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Still on the console, set the 32R to stagebox mode.
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Set your desired channels to network.
You should now be able to select mixes and go back to select the main mix.