Go into Audio Setup of Studio One and, if need be, delete the inputs and then re-add them. If you have a guitar amp or PA handy, go out of the Audiobox Mains on the back into the amp or PA. And don't overlook the Input knob, Mixer knob, and Main knob on the front. If it works directly going into an amp or PA, then your issue is software configuration--either in Windows or the app you're using.