Simple answer is yes. Maybe this will help: https://answers.presonus.com/31342/studio-one-v4-prime You need to setup your audio interface so that Studio One recognizes it. Then, when you create a new audio track in Studio One, you need to select the correct audio input channel. Then you turn on input monitoring and then the record button. You should see input signal coming in. Then select Record on the transport bar to record.