The problem is the device driver and how it's buffering audio into Studio One. Contact your device manufacturer for how they recommend you optimize your computer for audio recording. Each vendor has it's own recommended method of optimizations.
Consider reading this article we have on Latency which universally applies to any pro audio interface on Windows (or Mac).
We don't know what your interface is, assuming it's PCI, PCIe, 1394 or USB, the data path from the interface to Direct Memory must be clear of obstructions. You may have other devices on your system that are sharing resources with your device bus that are interfering with the audio stream.
Consider looking at your system to see if you have an DPC latencies that will affect realtime audio operations. A great tool for that is available here: http://www.thesycon.de/deu/latency_check.shtml
Read up on how to use that tool and how to dial your system in for better ASIO device performance. Most often the biggest culprits for pops and clicks with ASIO's on PCs are the video driver and the network card driver. Check your motherboard manual to find a USB controller that is not shared with another embedded device. ASUS Motherboards always have this in the PCI section of the manual.
If you're still having issues, you can turn to the user forum to get input from others on how they resolve the issue, and/or you can contact us at support.presonus.com so we can see if we can help you further.