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Maybe the reply I got from tech support will help you as well. It fixed my issue

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asked Oct 27, 2020 in Studio One 5 by stanthompson2 (5,910 points)

Technical SupportFriday at 12:04

Hello StanT, 

Thanks for letting me know which sound card, and for the NFO files. Sound Blasters are great for gaming, but can be problematic for Studio One. 

You can use a different Windows driver for the Sound Blaster. Please download and install ASIO4all(asio4all.org) and test to see if this makes a difference. Presonus in no way supports ASIO4all, but I have seen it help in situations when a device does not have its own ASIO driver, or is using Windows audio.

An ASIO driver provides a low-latency and high fidelity interface between a software application and a computer's sound card.

Make sure to install the ASIO4all offline settings, so you can enable the SB in the ASIO4all interface.

Then open Studio One > Options > Audio Setup and choose ASIO4all.  Start a new song in Studio One. Then in Song > Song Setup > Audio I/O setup, add your channels for input and out.

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