Drew,
First, we would suggest that you go ahead and update to the latest version of Studio One 3 Pro; version 3.3.3
Then it would be suggested that you double check the setup of your Alesis V25 inside of Studio One as directed by Alesis:
http://www.alesis.com/kb/article/1944#software
Re-test and if you are continuing to have issues, it may require you to contact Alesis as well as submitting a support request to PreSonus for further assistance.