Hi Nick,
You may want to open Studio One and go into Studio One>Preferences>Advanced>Services on your machine. You can then disable ARA (Melodyne), VST2.4, VST3, AU, and ReWire. You can then try to open your file. If it opens, you will want to turn those services back on, one at a time. Once you have identified the service type that is causing the problem, try disabling plug-ins in your project until you identify the one that is causing the issue. You can then contact the manufacturer of that plug-in for information on updates, installation tips, known issues, and compatibility. If you get stuck, please feel free to submit a support ticket under your my.presonus.com account and attach a crash report.
