5/16/2020 I am using Studio One 3 Professional . This addresses one specific setting but it may help. Start Studio One 3 (or your version) Select Studio One, then Options, then the Audio Setup tab The Options box in the lower left corner needs to be selected Select the Processing tab In the Process Precision drop down box, select Single (32 bit) – not Double (64 bit). My system is a 64 bit system but it still doesn't work. The sound should work. I do not have my Presonus Audio Box USB connected.
Step 3: You need to launch the song you plan to work on in Studio One. Once there you need to go the 'Song' drop down and select 'Song Setup'. You will now get the popup window. Select 'Audio I/O Setup' then Select the 'Outputs' tab. Note: The program defaults your audio outputs to Channel 1 & 2. Your mixer/computer is setup to send/receive your outputs from Channels 37 & 38. Set your output to those channels and your should have sound.
Thank you, this fixed the problem for me. I didn't spend 3 days but found your post after about 6 hours.
Why in the world Presonus would make these the default settings I can't possibly imagine?