No, you don't need DAW mode. If you have created a song in Studio One and used the SL 32 template, all routing is ready layouted for 32 channels plus a stereo return. You can move tracks in Studio One according to the layout you use in your console. Channel 2 is automatically routed to track 2 in Studio One. If you want to record to tack 2, input 2 on the console should be set to analog (the physical input of your mixer as input source).