I just checked to be sure. We are using different hardware. But if you're asking about passthrough, then no. I can play whatever in the line in but without another program doing the routing (it doesn't have to be a DAW) I don't get sound out of my monitors. I can get sound while watching movies, listening to mp3s, ect. So it does seem to need a program to route the sound for passthrough. But since various standalone guitar effects work just fine, It doesn't have to be Studio One. Sorry, I can't be more help.