The windows audio will need to be routed.
https://support.presonus.com/hc/en-us/articles/115005260746-Series-III-Rackmount-Playback-Audio-Routing-USB-AVB-
Using a template should help you in studio one.
https://support.presonus.com/hc/en-us/articles/360050925691-StudioLive-Series-III-Templates-How-to-get-Stereo-Playback-from-Studio-One